3PUW

Crystal Structure of an outward-facing MBP-Maltose transporter complex bound to ADP-AlF4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2009-05-10
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97965
Spacegroup name	P 1
Unit cell lengths	81.881, 97.344, 112.336
Unit cell angles	85.76, 79.42, 72.45

Refinement procedure

Resolution	19.990 - 2.300
R-factor	0.22194
Rwork	0.220
R-free	0.25480
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.003
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.5.0088)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.280
High resolution limit [Å]	2.200	2.200
Number of reflections	93125
<I/σ(I)>		1.7
Completeness [%]	60.9	16.6
Redundancy		2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	27 % PEG 400, 0.1 M HEPES, 10 mM magnesium chloride, 50 mM sodium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 293K, pH 7.5