3PUO

Crystal structure of dihydrodipicolinate synthase from Pseudomonas aeruginosa(PsDHDPS)complexed with L-lysine at 2.65A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	77
Detector technology	CCD
Collection date	2010-03-26
Detector	MARRESEARCH
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	81.340, 81.840, 81.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.270 - 2.650
R-factor	0.21773
Rwork	0.216
R-free	0.25546
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3noe
RMSD bond length	0.011
RMSD bond angle	1.450
Data reduction software	MOSFLM
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.800	2.680
High resolution limit [Å]	2.650	2.650
Number of reflections	16422
<I/σ(I)>	10	3.5
Completeness [%]	97.9	99

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	30% PEG 3350, 70mM Tris-HCl, 170mM MgCl2, 6% Propylene glycol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K