3PUJ

Crystal structure of the MUNC18-1 and SYNTAXIN4 N-Peptide complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-05
Detector	ADSC QUANTUM 315
Wavelength(s)	0.95369
Spacegroup name	P 41 21 2
Unit cell lengths	124.505, 124.505, 232.976
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.254 - 3.313
R-factor	0.2129
Rwork	0.211
R-free	0.25760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1DN1 Chain A
RMSD bond length	0.002
RMSD bond angle	0.559
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.1_357))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.430
High resolution limit [Å]	3.300	3.300
Rmerge	0.125	0.796
Number of reflections	26564
<I/σ(I)>	10.3	1.8
Completeness [%]	94.8	96.8
Redundancy	6	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	60% Tascimate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K