3PUB
Crystal structure of the Bombyx mori low molecular weight lipoprotein 7 (Bmlp7)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2009-11-10 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.855, 49.322, 54.790 |
| Unit cell angles | 93.26, 94.04, 103.47 |
Refinement procedure
| Resolution | 47.830 - 1.910 |
| R-factor | 0.19464 |
| Rwork | 0.193 |
| R-free | 0.23506 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.186 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.940 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Number of reflections | 31866 | |
| Completeness [%] | 96.8 | 95.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.05M 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (pH 7.5), 0.2M KCl, 28% pentaerythritol propoxylate (17/8, PO/OH), VAPOR DIFFUSION, HANGING DROP, temperature 298K |
