3PU7

Cu-Zn Tomato Chloroplast Superoxide Dismutase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2010-11-19
Detector	RIGAKU RAXIS HTC
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	58.246, 60.814, 79.693
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.169 - 1.800
R-factor	0.1756
Rwork	0.173
R-free	0.21390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3km2
RMSD bond length	0.006
RMSD bond angle	1.072
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.860
High resolution limit [Å]	1.800	1.800
Number of reflections	26639
<I/σ(I)>	13.1	6
Completeness [%]	98.7	100
Redundancy	3.4	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	0.1 M HEPES, 25% PEG 3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K