3PU1
Crystal Structure of a vesicular stomatitis virus nucleocapsid-polyG complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 293 |
Collection date | 2009-01-20 |
Detector | MAR 325 CCD |
Wavelength(s) | 0.98008 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 163.222, 233.189, 75.570 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.100 - 3.140 |
R-factor | 0.261 |
Rwork | 0.259 |
R-free | 0.30500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.354 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.6.1_357) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.100 | 3.260 |
High resolution limit [Å] | 3.140 | 3.140 |
Rmerge | 0.094 | |
Number of reflections | 42182 | |
Completeness [%] | 82.6 | 64.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.5 | 293 | 5.5 % PEG 3350, 200 mM sodium chloride, 100mM sodium acetate, pH 4.5, vapor diffusion, hanging drop, temperature 293.0K |