3PTJ

Structural and functional Analysis of Arabidopsis thaliana thylakoid lumen protein AtTLP18.3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-12-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97884
Spacegroup name	P 21 21 21
Unit cell lengths	46.977, 49.839, 76.672
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.600
Rwork	0.199
R-free	0.26900
Structure solution method	SAD
RMSD bond length	0.015
RMSD bond angle	1.600
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.690
High resolution limit [Å]	2.600	5.590	2.600
Rmerge	0.046	0.049	0.055
Number of reflections	11594
<I/σ(I)>	30.8	22.9	38.4
Completeness [%]	97.8	94.4	99.6
Redundancy	9.9	9.4	10.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	296	0.2M Sodium acetate trihydrate, 0.1M Sodium cacodylate, 25-30% (w/v) Polyethylene glycol 4000, 2mM TCEP , pH 6.0, VAPOR DIFFUSION, temperature 296K