3PTD
PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE C MUTANT D274S
Experimental procedure
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 1996-09-19 |
| Detector | XENTRONICS |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.200, 46.100, 161.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.700 - 2.200 |
| R-factor | 0.177 |
| Rwork | 0.177 |
| R-free | 0.26200 |
| Structure solution method | DIFFERENCE FOURIER |
| RMSD bond length | 0.006 |
| RMSD bond angle | 24.700 * |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.700 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.042 | 0.152 |
| Total number of observations | 35135 * | |
| Number of reflections | 16336 | |
| <I/σ(I)> | 14.3 | 4.6 |
| Completeness [%] | 71.2 | 43.3 |
| Redundancy | 2.15 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | 4 * | pH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4.5 (mg/ml) | |
| 2 | 1 | drop | HEPES | 18 (mM) | |
| 3 | 1 | drop | 0.0045 (%) | ||
| 4 | 1 | drop | PEG600 | 16.36 (%) | |
| 5 | 1 | drop | trisodium citrate | 0.09 (M) | |
| 6 | 1 | drop | 6.45 (mM) | ||
| 7 | 1 | reservoir | 1.5-2.25 (M) |
