3PT2
Structure of a viral OTU domain protease bound to Ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-19 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 146.010, 146.010, 58.140 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.793 - 2.500 |
| R-factor | 0.1699 |
| Rwork | 0.164 |
| R-free | 0.22420 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.906 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.800 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.093 | 0.401 |
| Number of reflections | 13116 | |
| <I/σ(I)> | 13.1 | 5.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.1 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 294 | 27% PEG4000, 0.1 M sodium acetate, 0.21 M ammonium sulfate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
