3PST
Crystal structure of PUL and PFU(mutate) domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BSRF BEAMLINE 1W2B |
Synchrotron site | BSRF |
Beamline | 1W2B |
Temperature [K] | 100 |
Wavelength(s) | 1.07 |
Spacegroup name | P 61 |
Unit cell lengths | 101.562, 101.562, 72.015 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.533 - 1.999 |
R-factor | 0.1904 |
Rwork | 0.188 |
R-free | 0.22590 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.902 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 87.955 |
High resolution limit [Å] | 1.999 |
Number of reflections | 28692 |
Completeness [%] | 100.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293.15 | 0.15M Potassium bromide,30% w/v PEG 2000, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |