Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.2398 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.268, 68.643, 62.537 |
| Unit cell angles | 90.00, 105.72, 90.00 |
Refinement procedure
| Resolution | 19.260 - 3.200 |
| R-factor | 0.1876 |
| Rwork | 0.185 |
| R-free | 0.24240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1z2w |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.377 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.700 | 3.370 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.106 | 0.475 |
| Number of reflections | 6568 | |
| <I/σ(I)> | 10.2 | 3.5 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 5.1 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.6 | 289 | 0.1M Tris, 0.2M NaCl, 20% PEG 3350, pH 8.6, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
