3PSK

Crystal Structure of the Spt6 Tandem SH2 Domain from Saccharomyces cerevisiae, Form Native Spt6 (1247-1451)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2008-03-01
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54178
Spacegroup name	P 21 21 21
Unit cell lengths	78.778, 105.176, 119.481
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.229 - 2.100
R-factor	0.1851
Rwork	0.184
R-free	0.21340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3psj
RMSD bond length	0.003
RMSD bond angle	0.687
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER (1.3.3)
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.180
High resolution limit [Å]	2.100	4.520	2.100
Rmerge	0.066	0.042	0.478
Number of reflections	58489
<I/σ(I)>	15.6
Completeness [%]	99.5	99.5	99.2
Redundancy	4.4	4.2	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	294	0.1M Tris pH5.5 25% PEG 3350 0.2M (NH4)2SO4 , VAPOR DIFFUSION, SITTING DROP, temperature 294K