3PSI

Crystal Structure of the Spt6 core domain from Saccharomyces cerevisiae, Form Spt6(239-1451)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-05-08
Detector	ADSC QUANTUM 315
Wavelength(s)	1.10000
Spacegroup name	P 31 2 1
Unit cell lengths	118.747, 118.747, 214.408
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.670 - 3.300
R-factor	0.2687
Rwork	0.265
R-free	0.30840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.577
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.420
High resolution limit [Å]	3.300	7.100	3.300
Rmerge	0.058	0.032	0.569
Number of reflections	27025
<I/σ(I)>	15.4
Completeness [%]	99.5	96.8	99.9
Redundancy	6.7	6.7	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	286	0.1M Na-MES 13% PEG 4000 0.2 M MgCl2 12% Glycerol , pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 286K