3PSH
Classification of a Haemophilus influenzae ABC transporter HI1470/71 through its cognate molybdate periplasmic binding protein MolA (MolA bound to Molybdate)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-12-07 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 1.54, 1.00 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 123.295, 123.295, 103.037 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.940 - 1.500 |
R-factor | 0.2976 |
Rwork | 0.295 |
R-free | 0.31670 |
Structure solution method | SAD |
RMSD bond length | 0.031 |
RMSD bond angle | 2.384 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHELX |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 1.500 |
Number of reflections | 138477 |
Completeness [%] | 96.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 277 | 24% PEG2000 MME, 0.1 M sodium acetate pH 4.5, 0.2 M ammonium sulfate, 12% glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 277K |