3PS0
The structure of the CRISPR-associated protein, csa2, from Sulfolobus solfataricus
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-24 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.95369, 1.04013, 1.03982, 0.96109 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.642, 120.876, 158.056 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.750 - 2.000 |
| R-factor | 0.185 |
| Rwork | 0.183 |
| R-free | 0.22100 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.930 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.041 | 0.457 |
| Number of reflections | 96002 | |
| <I/σ(I)> | 34.6 | 2.7 |
| Completeness [%] | 98.7 | 100 |
| Redundancy | 5.4 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 295 | 2.0 M SODIUM FORMATE, 0.1 M SODIUM ACETATE PH 4.0, 0.2 M SODIUM THIOCYANATE, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
