3PRU

Crystal Structure of Phycobilisome 32.1 kDa linker polypeptide, phycocyanin-associated, rod 1 (fragment 14-158) from Synechocystis sp. PCC 6803, Northeast Structural Genomics Consortium Target SgR182A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-02
Detector	ADSC QUANTUM 4
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	74.354, 91.532, 124.673
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.770 - 2.677
R-factor	0.195
Rwork	0.193
R-free	0.24100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ohw
RMSD bond length	0.008
RMSD bond angle	1.172
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	BALBES
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.101	0.512
Number of reflections	45882
<I/σ(I)>	26.2	4.7
Completeness [%]	100.0	100
Redundancy	4.8	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5). Reservoir solution: 3.7M NaCl, 0.1M HEPES-Na, VAPOR DIFFUSION, HANGING DROP, temperature 293KK