3PRP
Structural analysis of a viral OTU domain protease from the Crimean-Congo Hemorrhagic Fever virus in complex with human ubiquitin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-08-23 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.978 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 79.316, 105.845, 113.032 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 77.260 - 1.699 |
R-factor | 0.174 |
Rwork | 0.172 |
R-free | 0.21200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3prm |
RMSD bond length | 0.012 |
RMSD bond angle | 1.351 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.1.4) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 77.260 | 1.760 |
High resolution limit [Å] | 1.699 | 1.699 |
Rmerge | 0.055 | 0.186 |
Number of reflections | 50853 | |
<I/σ(I)> | 2.8 | |
Completeness [%] | 96.5 | 74.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 22-28% PEG 8000, 100 mM Na cacodylate pH 6.5, 100-250 mM Mg acetate, and 2% n-Octyl- -D-glucoside, VAPOR DIFFUSION, HANGING DROP, temperature 298K |