3PRG

LIGAND BINDING DOMAIN OF HUMAN PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1998-06-22
Detector	RIGAKU
Spacegroup name	P 43 21 2
Unit cell lengths	67.250, 67.250, 156.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.900
R-factor	0.209
Rwork	0.209
R-free	0.27100
Structure solution method	MIRAS
RMSD bond length	0.007
RMSD bond angle	19.000 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (0.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.070 *
Number of reflections	8069
<I/σ(I)>	11	1.8
Completeness [%]	96.0	98
Redundancy	2.3	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	9.5		pH 9.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	3 (mg/ml)
2	1	reservoir	sodium formate	3.4 (M)
3	1	reservoir	Tris-HCl	0.1 (M)
4	1	reservoir	PEG6000	1 (%)