3PRD
Structural analysis of protein folding by the Methanococcus jannaschii chaperone FKBP26
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-10-01 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 137.950, 137.950, 114.570 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 3.300 |
R-factor | 0.3153 |
Rwork | 0.315 |
R-free | 0.32790 |
Structure solution method | MAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.056 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 25.000 |
High resolution limit [Å] | 3.300 |
Number of reflections | 8151 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | hanging drop | 298 | 1.5 M (NH4)2SO4, pH 100 mM Tris, pH 8.0, hanging drop, temperature 298K |