3PRC

PHOTOSYNTHETIC REACTION CENTER FROM RHODOPSEUDOMONAS VIRIDIS (QB-DEPLETED)

Experimental procedure

Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	263
Detector technology	IMAGE PLATE
Collection date	1993-07
Detector	MARRESEARCH
Wavelength(s)	0.92, 1.0
Spacegroup name	P 43 21 2
Unit cell lengths	223.500, 223.500, 113.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.400
R-factor	0.178
Rwork	0.178
R-free	0.21500
Structure solution method	DIFFERENCE FOURIER
Starting model (for MR)	2prc
RMSD bond length	0.011
RMSD bond angle	23.600 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((ROTAVATA)
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.700	2.460
High resolution limit [Å]	2.400	2.400
Rmerge	0.087	0.287
Total number of observations	233535 *
Number of reflections	87694 *
<I/σ(I)>	6.9	2.4
Completeness [%]	79.4	61.1 *
Redundancy	2.6	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6		pH 6.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	ammonium sulfate	1.5 (M)
2	1	reservoir	ammonium sulfate	3 (M)	or 2.6M or 2.4M