3PR7
Multi-functional and mechanosensitive receptor binding activity of the Moraxella catarrhalis adhesin UspA1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Detector technology | CCD |
| Collection date | 2010-09-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 63 |
| Unit cell lengths | 118.219, 118.219, 176.570 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.600 - 2.940 |
| R-factor | 0.2033 |
| Rwork | 0.203 |
| R-free | 0.21620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.040 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 102.380 |
| High resolution limit [Å] | 2.930 |
| Rmerge | 0.116 |
| Number of reflections | 29914 |
| <I/σ(I)> | 16.3 |
| Completeness [%] | 99.9 |
| Redundancy | 12.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.1 M MES, 0.2 M sodium dihydrogen phosphate, 0.2 M potassium dihydrogen phosphate, 1 M sodium chloride, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
