3PR5

Dpo4 Y12A mutant incorporating ADP opposite template dT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2007-06-04
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	95.607, 101.993, 52.288
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.293 - 2.400
R-factor	0.2291
Rwork	0.228
R-free	0.26470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jx4
RMSD bond length	0.009
RMSD bond angle	1.316
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.460
High resolution limit [Å]	2.400	2.400
Number of reflections	19626
Completeness [%]	95.1	81.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298.15	15% PEG 3350, 0.15M CaAc2, 0.1M Hepes pH 7.0, 2.5% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 298.15K