3PQK
Crystal Structure of the transcriptional repressor BigR from Xylella fastidiosa
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE W01B-MX2 |
Synchrotron site | LNLS |
Beamline | W01B-MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-09-20 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97954, 0.9795, 0.9797, 0.9282 |
Spacegroup name | P 1 |
Unit cell lengths | 34.230, 34.230, 140.830 |
Unit cell angles | 90.03, 90.00, 120.03 |
Refinement procedure
Resolution | 18.490 - 2.090 |
R-factor | 0.2111 |
Rwork | 0.209 |
R-free | 0.24220 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.030 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHARP |
Refinement software | BUSTER (2.9.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 2.220 |
High resolution limit [Å] | 2.090 | 2.090 |
Number of reflections | 30801 | |
<I/σ(I)> | 11.33 | 2.8 |
Completeness [%] | 94.0 | 92.4 |
Redundancy | 2.2 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |