3PQJ
Crystal Structure of the transcriptional repressor BigR from Xylella fastidiosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.4333 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.590, 47.840, 54.700 |
| Unit cell angles | 90.03, 89.96, 105.34 |
Refinement procedure
| Resolution | 20.860 - 2.480 |
| R-factor | 0.1967 |
| Rwork | 0.194 |
| R-free | 0.24990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.170 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.9.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.630 |
| High resolution limit [Å] | 2.480 | 2.480 |
| Number of reflections | 13185 | |
| <I/σ(I)> | 13.22 | 3.97 |
| Completeness [%] | 93.8 | 83.8 |
| Redundancy | 3.88 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
