3PQI
Crystal structure of the bacteriophage phi92 membrane-piercing protein gp138
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-07 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 65.144, 65.144, 329.375 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 55.607 - 2.642 |
| R-factor | 0.2127 |
| Rwork | 0.211 |
| R-free | 0.25010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.794 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.610 | 2.780 |
| High resolution limit [Å] | 2.640 | 2.642 |
| Rmerge | 0.084 | 0.517 |
| Number of reflections | 8304 | |
| <I/σ(I)> | 18.4 | |
| Completeness [%] | 99.8 | 98.1 |
| Redundancy | 10.5 | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 10 mg/ml 10% - 12% PEG 2K 100mM - 200mM MgCl2 100mM Tris pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
