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3PPN

Structures of the substrate-binding protein provide insights into the multiple compatible solutes binding specificities of Bacillus subtilis ABC transporter OpuC

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSSRF BEAMLINE BL17U
Synchrotron siteSSRF
BeamlineBL17U
Temperature [K]100
Detector technologyCCD
Collection date2010-07-14
DetectorMARMOSAIC 225 mm CCD
Wavelength(s)0.9194
Spacegroup nameP 21 21 21
Unit cell lengths89.275, 89.667, 90.387
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution29.980 - 2.300
R-factor0.2179
Rwork0.217
R-free0.23910
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1sw2
RMSD bond length0.016
RMSD bond angle1.406
Data reduction softwareHKL-2000
Data scaling softwareSCALA
Phasing softwareMOLREP
Refinement softwareREFMAC (5.5.0109)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]30.0002.380
High resolution limit [Å]2.3002.300
Number of reflections32670
Completeness [%]100.0100
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP728932%(v/v) Jeffamine M-600, 0.1M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K

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