3PPH
Crystal structure of the Candida albicans methionine synthase by surface entropy reduction, threonine variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.11587 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 163.636, 98.080, 162.586 |
| Unit cell angles | 90.00, 131.10, 90.00 |
Refinement procedure
| Resolution | 47.664 - 2.800 |
| R-factor | 0.2132 |
| Rwork | 0.210 |
| R-free | 0.27560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ppc |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.153 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.664 | 47.664 | 2.950 |
| High resolution limit [Å] | 2.800 | 8.850 | 2.800 |
| Rmerge | 0.109 | 0.039 | 0.659 |
| Number of reflections | 47736 | ||
| <I/σ(I)> | 9.2 | 13.9 | 1.2 |
| Completeness [%] | 99.8 | 99.4 | 99.9 |
| Redundancy | 3.6 | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 25% (w/v) PEG-MME 2000, 75 mM KSCN, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
