3PPF

Crystal structure of the Candida albicans methionine synthase by surface entropy reduction, alanine variant without zinc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-06-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97740
Spacegroup name	P 21 21 21
Unit cell lengths	76.862, 98.814, 100.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.407 - 2.300
R-factor	0.1844
Rwork	0.181
R-free	0.25320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ppc
RMSD bond length	0.007
RMSD bond angle	1.047
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.340
High resolution limit [Å]	2.300	6.240	2.300
Rmerge	0.087	0.037	0.545
Number of reflections	35447
<I/σ(I)>	9.9
Completeness [%]	99.9	99.7	99.3
Redundancy	6.4	5.9	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	293	50 mM NaI, 27% (w/v) PEG 3350, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K