3PPE
Crystal structure of chicken VE-cadherin EC1-2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4C |
Synchrotron site | NSLS |
Beamline | X4C |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-04-09 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.97885 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 99.973, 99.973, 105.987 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 72.740 - 2.100 |
R-factor | 0.18692 |
Rwork | 0.184 |
R-free | 0.24189 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2a4e |
RMSD bond length | 0.017 |
RMSD bond angle | 1.666 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 31991 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 14.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293.15 | 18% (w/v) PEG 8,000, 200mM Calcium acetate, 100mM sodium cacodylate pH6.5., VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |