3PPA
Structure of the Dusp-Ubl domains of Usp15
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97921 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 74.170, 74.170, 194.520 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.800 - 2.350 |
| R-factor | 0.2629 |
| Rwork | 0.262 |
| R-free | 0.28520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3jyu |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.130 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.390 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Number of reflections | 13829 | |
| <I/σ(I)> | 31.328 | 2.21 |
| Completeness [%] | 98.9 | 97.6 |
| Redundancy | 12.7 | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 291.1 | 0.5 M ammonium formate, 1.0 M lithium sulfate, 0.1 M citrate pH 5.6; 1 uM dispase added, VAPOR DIFFUSION, SITTING DROP, temperature 291.1K |
