3PP2

Crystal structure of the pleckstrin homology domain of ArhGAP27

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-07
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97625
Spacegroup name	P 31 2 1
Unit cell lengths	98.243, 98.243, 66.909
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 1.421
R-factor	0.172
Rwork	0.171
R-free	0.19190
RMSD bond length	0.017
RMSD bond angle	1.669
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.440
High resolution limit [Å]	1.420	3.850	1.420
Rmerge	0.059	0.028	0.872
Number of reflections	136029
<I/σ(I)>	12.8
Completeness [%]	99.8	100	95.9
Redundancy	8.5	11.2	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	1.4 M sodium citrate, 0.1 M HEPES, 1:100 w/w endoproteinase Glu-C V8, pH 7.5, vapor diffusion, hanging drop, temperature 293K