3PP2
Crystal structure of the pleckstrin homology domain of ArhGAP27
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-07 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97625 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 98.243, 98.243, 66.909 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 1.421 |
R-factor | 0.172 |
Rwork | 0.171 |
R-free | 0.19190 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.669 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 1.440 |
High resolution limit [Å] | 1.420 | 3.850 | 1.420 |
Rmerge | 0.059 | 0.028 | 0.872 |
Number of reflections | 136029 | ||
<I/σ(I)> | 12.8 | ||
Completeness [%] | 99.8 | 100 | 95.9 |
Redundancy | 8.5 | 11.2 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 1.4 M sodium citrate, 0.1 M HEPES, 1:100 w/w endoproteinase Glu-C V8, pH 7.5, vapor diffusion, hanging drop, temperature 293K |