3POQ
Crystal structure of E.coli OmpF porin in lipidic cubic phase: space group H32, small unit cell
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Collection date | 2009-11-24 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.00 |
Spacegroup name | H 3 2 |
Unit cell lengths | 77.310, 77.310, 330.360 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.352 - 1.900 |
R-factor | 0.2295 |
Rwork | 0.227 |
R-free | 0.28000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2omf |
RMSD bond length | 0.009 |
RMSD bond angle | 1.144 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.16) |
Phasing software | PHASER (2.1.4) |
Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 19.352 | 19.352 | 2.000 |
High resolution limit [Å] | 1.900 | 6.010 | 1.900 |
Rmerge | 0.172 | 0.088 | 0.430 |
Rmeas | 0.097 | 0.494 | |
Rpim | 0.039 | 0.237 | |
Total number of observations | 4424 | 16313 | |
Number of reflections | 27555 | ||
<I/σ(I)> | 6.3 | 11.5 | 2.4 |
Completeness [%] | 91.2 | 83.5 | 95.3 |
Redundancy | 4.6 | 5.1 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.6 | 296 | 0.1M sodium acetate, 2.7M lithium nitrate, 2.0M potassium thiocyanate, pH 4.6, lipidic sponge phase, temperature 296K |