3POQ

Crystal structure of E.coli OmpF porin in lipidic cubic phase: space group H32, small unit cell

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Collection date	2009-11-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00
Spacegroup name	H 3 2
Unit cell lengths	77.310, 77.310, 330.360
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.352 - 1.900
R-factor	0.2295
Rwork	0.227
R-free	0.28000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2omf
RMSD bond length	0.009
RMSD bond angle	1.144
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.16)
Phasing software	PHASER (2.1.4)
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.352	19.352	2.000
High resolution limit [Å]	1.900	6.010	1.900
Rmerge	0.172	0.088	0.430
Rmeas		0.097	0.494
Rpim		0.039	0.237
Total number of observations		4424	16313
Number of reflections	27555
<I/σ(I)>	6.3	11.5	2.4
Completeness [%]	91.2	83.5	95.3
Redundancy	4.6	5.1	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.6	296	0.1M sodium acetate, 2.7M lithium nitrate, 2.0M potassium thiocyanate, pH 4.6, lipidic sponge phase, temperature 296K