3POA

Structural and functional analysis of phosphothreonine-dependent FHA domain interactions

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.1
Synchrotron site	SRS
Beamline	PX14.1
Temperature [K]	100
Detector technology	CCD
Wavelength(s)	1.2
Spacegroup name	C 2 2 21
Unit cell lengths	57.016, 74.425, 63.016
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.050 - 2.010
R-factor	0.18167
Rwork	0.179
R-free	0.23706
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.285
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall
Low resolution limit [Å]	19.050
High resolution limit [Å]	2.010
Number of reflections	8693

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP			VAPOR DIFFUSION, HANGING DROP