3POA
Structural and functional analysis of phosphothreonine-dependent FHA domain interactions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Wavelength(s) | 1.2 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 57.016, 74.425, 63.016 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.050 - 2.010 |
| R-factor | 0.18167 |
| Rwork | 0.179 |
| R-free | 0.23706 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.285 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 19.050 |
| High resolution limit [Å] | 2.010 |
| Number of reflections | 8693 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | VAPOR DIFFUSION, HANGING DROP |
