3PNW

Crystal Structure of the tudor domain of human TDRD3 in complex with an anti-TDRD3 FAB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2010-08-18
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97924
Spacegroup name	P 1
Unit cell lengths	76.708, 93.726, 159.932
Unit cell angles	80.96, 82.82, 90.06

Refinement procedure

Resolution	30.000 - 2.050
R-factor	0.224
Rwork	0.223
R-free	0.26350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	pdb entries 2HFF (modified on FFAS03 server) and 3PMT
RMSD bond length	0.014
RMSD bond angle	1.267
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.120
High resolution limit [Å]	2.050	4.420	2.050
Rmerge	0.086	0.044	0.414
Number of reflections	266201
<I/σ(I)>	6.4
Completeness [%]	97.4	99	93.1
Redundancy	2.5	2.6	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	16% PEG-3350, 0.05M citric acid, 0.05M bis-tris propane, vapor diffusion, sitting drop, temperature 293K