3PNT
Crystal Structure of the Streptococcus pyogenes NAD+ glycohydrolase SPN in complex with IFS, the Immunity Factor for SPN
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | NOIR-1 |
| Wavelength(s) | 0.97892, 0.97938, 0.99510 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 198.817, 57.892, 89.699 |
| Unit cell angles | 90.00, 107.20, 90.00 |
Refinement procedure
| Resolution | 42.720 - 2.800 |
| R-factor | 0.21166 |
| Rwork | 0.208 |
| R-free | 0.27811 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.193 |
| Refinement software | REFMAC (5.5.0093) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 42.720 |
| High resolution limit [Å] | 2.800 |
| Number of reflections | 24309 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | PEG 8000, NaCl, Tris (8.0), vapor diffusion, hanging drop, temperature 298K |
