3PNN
The crystal structure of a glycosyltransferase from Porphyromonas gingivalis W83
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97942 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 109.038, 109.038, 124.637 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.153 - 1.901 |
| R-factor | 0.1667 |
| Rwork | 0.166 |
| R-free | 0.18340 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.078 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.200 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.108 | 0.649 |
| Number of reflections | 34856 | |
| <I/σ(I)> | 43.9 | 4.1 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 11.7 | 11.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 1M Sodium Citrate Tribasic, 0.1M Sodium Cacodylate:HCl, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
