3PMZ
Crystal Structure of the Complex of Acetylcholine Binding Protein and d-tubocurarine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-15 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 142.737, 194.042, 101.615 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 114.710 - 2.440 |
| R-factor | 0.232 |
| Rwork | 0.228 |
| R-free | 0.29400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2byn |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.866 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 114.710 | 2.640 |
| High resolution limit [Å] | 2.440 | 2.440 |
| Rmerge | 0.109 | |
| Number of reflections | 101910 | |
| <I/σ(I)> | 41.3 | |
| Completeness [%] | 96.9 | 93.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 295 | 10% PEG 4000, 0.25M magnesium chloride, 0.1M Tris buffer pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K |
