3PMO
The structure of LpxD from Pseudomonas aeruginosa at 1.3 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | H 3 |
| Unit cell lengths | 106.190, 106.190, 93.380 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.850 - 1.300 |
| R-factor | 0.16546 |
| Rwork | 0.164 |
| R-free | 0.18532 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.171 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.850 | 1.370 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.078 | 0.644 |
| Number of reflections | 96539 | |
| <I/σ(I)> | 14.1 | 3.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 9 | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 10 mM Tris HCl, 1mM DTT, 500mM NaCl, 2 uL of 100 mM Tris, 1.5 M lithium sulfate, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
