3PMK
Crystal structure of the Vesicular Stomatitis Virus RNA free nucleoprotein/phosphoprotein complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-13 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9334 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 74.560, 171.970, 239.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.970 - 3.030 |
| R-factor | 0.24561 |
| Rwork | 0.244 |
| R-free | 0.27791 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2GIC chain E |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.894 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 59.970 | 59.970 | 3.200 |
| High resolution limit [Å] | 3.030 | 9.590 | 3.030 |
| Number of reflections | 54333 | ||
| <I/σ(I)> | 5.6 | ||
| Completeness [%] | 89.9 | 99.4 | 80.7 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 4% (w/v) PEG 4000, 0.1M sodium acetate trihydrate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
