3PM7
Crystal Structure of EF_3132 protein from Enterococcus faecalis at the resolution 2A, Northeast Structural Genomics Consortium Target EfR184
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-09 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 65 |
| Unit cell lengths | 78.851, 78.851, 59.384 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.600 - 2.001 |
| R-factor | 0.21 |
| Rwork | 0.208 |
| R-free | 0.25000 |
| Structure solution method | SAD |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.062 | 0.494 |
| Number of reflections | 25009 | |
| <I/σ(I)> | 24.1 | 1.4 |
| Completeness [%] | 89.6 | 60.7 |
| Redundancy | 7.2 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | macrobatch under oil | 4.2 | 293 | Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5). Reservoir solution:Li2So4 0.1M, Sodium Citrate 0.1M, PEG 20000 12% (w/v), macrobatch under oil, temperature 293KK |
