3PM2
Crystal structure of a novel type of odorant binding protein from Anopheles gambiae belonging to the c+ class
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-05-08 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97625 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 36.289, 36.289, 263.541 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.990 - 1.800 |
R-factor | 0.1887 |
Rwork | 0.187 |
R-free | 0.22720 |
Structure solution method | remote SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.020 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELXD |
Refinement software | BUSTER (2.9.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.300 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.167 | 0.550 |
Number of reflections | 17578 | |
<I/σ(I)> | 19.3 | 4 |
Completeness [%] | 99.9 | 100 |
Redundancy | 11.5 | 8.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | Protein 12 mg/ml, 30-35% PEG5000 MME, 0.1 M sodium acetate pH 5.7-6.3, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | Protein 12 mg/ml, 30-35% PEG5000 MME, 0.1 M sodium acetate pH 5.7-6.3, VAPOR DIFFUSION, SITTING DROP, temperature 298K |