3PM1

Structure of QacR E90Q bound to Ethidium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	298
Collection date	2005-04-23
Wavelength(s)	1.0
Spacegroup name	P 62
Unit cell lengths	104.148, 104.148, 98.564
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.090 - 2.800
R-factor	0.229
Rwork	0.229
R-free	0.28000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3RB2
RMSD bond length	0.009
RMSD bond angle	1.100
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.000	2.980
High resolution limit [Å]	2.800	2.800
Number of reflections	13889
Completeness [%]	92.0	92

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	298	ammonium sulfate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K