3PLV
Structure of Hub-1 protein in complex with Snu66 peptide (HINDII)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2006-05-09 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.05 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 36.720, 83.570, 35.110 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.780 - 1.900 |
| R-factor | 0.20301 |
| Rwork | 0.201 |
| R-free | 0.24177 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ubi |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.191 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.136 | |
| Number of reflections | 8959 | |
| <I/σ(I)> | 9.96 | |
| Completeness [%] | 99.4 | 97.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.9 | 290 | 0.2M Ammonium Iodide, 20% PEG 3350, pH 6.9, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
