3PLT
Crystal structure of Lsp1 from Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-21 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.979700 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 273.160, 38.753, 75.509 |
| Unit cell angles | 90.00, 99.35, 90.00 |
Refinement procedure
| Resolution | 37.510 - 2.900 |
| R-factor | 0.239 |
| Rwork | 0.236 |
| R-free | 0.29600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.130 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.106 | 0.896 |
| Number of reflections | 17725 | |
| <I/σ(I)> | 18.75 | 1.91 |
| Completeness [%] | 99.6 | 99.8 |
| Redundancy | 6.8 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 11 | 293 | 0.2M NaCl, 20mM phenol, 20% PEG 8000, 0.1M CAPS, pH 11, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
