3PLS
RON in complex with ligand AMP-PNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 41 |
| Unit cell lengths | 98.631, 98.631, 45.297 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.320 - 2.240 |
| R-factor | 0.2352 |
| Rwork | 0.231 |
| R-free | 0.28970 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.278 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 98.530 | 2.300 |
| High resolution limit [Å] | 2.240 | 2.240 |
| Rmerge | 0.052 | 0.509 |
| Number of reflections | 21232 | |
| Completeness [%] | 89.0 | 54.6 |
| Redundancy | 4.6 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 20% PEG2K and 2mM MgCl2, pH 7.5, VAPOR DIFFUSION |
