3PLH
urate oxidase under 1.5 MPa / 15 bars pressure of equimolar mixture xenon : nitrous oxide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 2008-12-04 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.0 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 80.275, 96.252, 105.513 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.980 - 1.800 |
R-factor | 0.16251 |
Rwork | 0.161 |
R-free | 0.18222 |
Structure solution method | RIGID BODY |
Starting model (for MR) | 2iba |
RMSD bond length | 0.014 |
RMSD bond angle | 1.481 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 37397 | |
<I/σ(I)> | 20.9 | 3 |
Completeness [%] | 97.8 | 90.3 |
Redundancy | 3.7 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH | 8.5 | 298 | 10-15 mg/ml urate oxidase, 2mg/ml 8-azaxanthine, 50 mM Tris/HCl pH 8.5, 5-8 % PEG 8000, 0-0.05 M NaCl, BATCH, temperature 298K |