3PL9
Crystal structure of spinach minor light-harvesting complex CP29 at 2.80 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-15 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.99985 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 68.793, 68.793, 425.778 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.560 - 2.800 |
| R-factor | 0.282 |
| Rwork | 0.282 |
| R-free | 0.29300 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.700 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.114 | 0.586 |
| Number of reflections | 14714 | |
| <I/σ(I)> | 18.5 | 5.6 |
| Completeness [%] | 92.2 | 40.9 |
| Redundancy | 11.9 | 11.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 100mM MES, pH 6.5, 2.80% (w/v) HTG, 19% (w/v) PEG 1000, 0.1M sodium chloride, 0.19M ammonium sulfate , VAPOR DIFFUSION, SITTING DROP, temperature 291K |
