3PL5
Fatty acid binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2007-08-10 |
| Detector | OXFORD ONYX CCD |
| Wavelength(s) | 1.54056 |
| Spacegroup name | P 41 |
| Unit cell lengths | 42.304, 42.304, 151.314 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.280 - 2.040 |
| R-factor | 0.18579 |
| Rwork | 0.182 |
| R-free | 0.25342 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pzx |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.683 |
| Data reduction software | CrysalisPro |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.280 | 2.150 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.096 | 0.210 |
| Number of reflections | 16904 | |
| <I/σ(I)> | 6.5489 | 3.31 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.42 | 5.51 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 0.1M MES, 20% PEG 3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
