3PL0
Crystal structure of a bsmA homolog (Mpe_A2762) from Methylobium petroleophilum PM1 at 1.91 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-09-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9537,0.9796,0.9793 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.508, 63.203, 104.504 |
Unit cell angles | 90.00, 97.80, 90.00 |
Refinement procedure
Resolution | 28.923 - 1.910 |
R-factor | 0.1715 |
Rwork | 0.169 |
R-free | 0.21090 |
Structure solution method | MAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.512 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELX |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 28.923 | 28.923 | 1.980 |
High resolution limit [Å] | 1.910 | 4.100 | 1.910 |
Rmerge | 0.042 | 0.022 | 0.543 |
Number of reflections | 43643 | 8095 | 8670 |
<I/σ(I)> | 10.4 | 26.4 | 1.9 |
Completeness [%] | 96.6 | 92.6 | 97.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.90M lithium chloride, 6.00% polyethylene glycol 6000, 0.1M HEPES pH 6.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |