3PKL
Urate oxidase under 0.8 MPa / 8 bars pressure of xenon
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM16 |
Synchrotron site | ESRF |
Beamline | BM16 |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2008-07-11 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.979 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 80.276, 96.327, 105.495 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.990 - 1.749 |
R-factor | 0.17409 |
Rwork | 0.173 |
R-free | 0.19586 |
Structure solution method | RIGID BODY |
Starting model (for MR) | 2iba |
RMSD bond length | 0.013 |
RMSD bond angle | 1.398 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.810 |
High resolution limit [Å] | 1.749 | 1.749 |
Number of reflections | 37664 | |
<I/σ(I)> | 24.2 | 3 |
Completeness [%] | 90.7 | 95.1 |
Redundancy | 5.3 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH | 8.5 | 298 | 10-15 mg/ml Urate oxidase, 2 mg/ml 8-azaxanthine, 50 mM Tris/HCl pH 8.5, 5-8 % PEG 8000, 0-0.05 M NaCl, BATCH, temperature 298K |